Mechanical properties of sciatic nerve injury after repaired with poly(lactic acid-glycolic acid) scaffold

doi:10.3969/j.issn.2095-4344.2017.06.017

Abstract

Abstract:

BACKGROUND: The tensile and stress-relaxation mechanical properties of sciatic nerve injury after repaired with autologous nerve and poly(lactic acid-glycolic acid) (PLGA) scaffold are rarely reported.
OBJECTIVE: To analyze the tensile and stress relaxation characteristics of sciatic nerve injury after the transplantation of autologous nerve and PLGA scaffold.
METHODS: Sixty sciatic nerves were extracted from the fresh cadavers dead within 24 hours, processed into 35 mm samples, and were then randomly divided into three groups. The nerve samples in control group received no intervention; the nerves in artificial and autologous groups were modeled into 20 mm defects, followed by repaired with PLGA scaffold and autologous nerve, respectively. Afterwards, the tension and stress-relaxation tests were performed in each group.
RESULTS AND CONCLUSION: The stress in each group descended fast at the first 600 seconds, then descended slowly and was closed to the horizontal level until 7 200 seconds, and the stress-relaxation curves in each group were in logarithmic decrease. The order of the elastic limit load, elastic limit stress, maximum load, maximum stress, elastic limit strain and maximum strain during tension was as follows: control group > artificial group > autologous group (P < 0.05). Our results indicate that the PLGA scaffold holds good tension and stress-relaxation properties, which meets the mechanical requirements of the biomaterials used for sciatic nerve repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Nerve Regeneration, Sciatic Nerve, Tissue Engineering

CLC Number:

R318

Li Zheng-wei, Lv Xue-man, Li Xin-ying, Li Ya-jun, Luo Min. Mechanical properties of sciatic nerve injury after repaired with poly(lactic acid-glycolic acid) scaffold[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(6): 917-922.

Figures/Tables 4

References

[1]Urita Y,Komuro H,Chen G,et al.Evaluation of diaphragmatic hernia repair using PLGA mesh-collagen sponge hybrid scaffold: an experimental study in a rat model.Pediatr Surg Int.2008;24(9):1041-1045.

[2]Jung Y,Park MS,Lee JW,et al.Cartilage regeneration with highly-elastic three-dimensional scaffolds prepared from biodegradable poly(L-lactide-co-epsilon-caprolactone). Biomaterials.2008;29(35):4630-4636.

[3]Aravind A,Nair R,Raveendran S,et al.Aptamer conjugated paclitaxel and magnetic fluid loaded fluorescently tagged PLGA nanoparticles for targeted cancer therapy.J Magn Magn Mater.2013;344(344):116-123.

[4]Jagani HV,Josyula VR,Palanimuthu VR,et al.Improvement of therapeutic efficacy of PLGA nanoformulation of siRNA targeting anti-apoptotic Bcl-2 through chitosan coating.Eur J Pharm Sci.2013;48(4-5):611-668.

[5]Cui Y,Xu Q,Chow PK,et al.Transferrin-conjugated magnetic silica PLGA nanoparticles loaded with doxorubicin and paclitaxel for brain glioma treatment.Biomaterials. 2013; 34(33):8511-8520.

[6]Lin L,Gao H,Dong Y.Bone regeneration using afreeze-dried 3D gradient-structured scaffold incor-porating OIC-A006-oaded PLGA microspheresbased on beta-TCP/PLGA. J Mater Sci Mater Med.2015;26(1):5327．

[7]林利,王伟莉,黄继锋,等.彩色多普勒超声对PRGD复合神经导管修复周围神经缺损的评价[J].华南国防医学杂志, 2014,(6): 570-572.

[8]门永芝,於子卫.生物材料构建神经导管修复周围神经损伤的研究进展[J].听力学及言语疾病杂志,2014,22(6):654-658.

[9]王华松,吴刚,黄继锋,等.新型仿生人工神经导管修复周围神经缺损的初步临床观察[J].中国临床解剖学杂志, 2014,32(6): 735-738.

[10]Jeon S,Jha MK,Ock J,et al. Role of lipocalin-2-chemokineaxis in the development of neuropathic pain followingperipheral nerve injury.J Biol Chem.2013;288(33):24116-24127.

[11]Arslantunali D,Budak G,Hasirci V.Multiwalled CNT-pHEMAcomposite conduit for peripheral nerve repair.J Biomed MaterRes A.2014;102(3):828-841.

[12]Benzina O,Szabo V,Lucas O,et al.Changes induced byperipheral nerve injury in the morphology and nanomechanicsof sensory neurons.J Biomed Opt.2013; 18(10):106014.

[13]Huang J,Zhang Y,Lu L,et al.Electrical stimulationaccelerates nerve regeneration and functional recovery indelayed peripheral nerve injury in rats.Eur J Neurosci.2013; 38(12): 3691-3701.

[14]张伟,岳靓,易红蕾,等.自组装多肽凝胶联合可降解神经导管修复长节段周围神经损伤[J].中国矫形外科杂志, 2011,19(24): 2083-2087.

[15]刘勇,侯春林.神经导管研究进展[J].国际骨科学杂志, 2010, 31(5): 279-221.

[16]赵文.新型复合材料神经导管促进周围神经再生的研究[D].上海:上海交通大学,2007.

[17]张伟,侯春林.神经移植术修复周围神经损伤[J].国外医学(骨科学分册),2002,23(2):98-101.

[18]Hsieh SC,Tang CM,Huang WT,et al.Comparison between twodifferent methods of immobilizing NGF in poly(DL-lacticacid-co-glycolic acid) conduit for peripheral nerveregeneration by EDC/NHS/MES and genipin.J Biomed MaterRes A.2011;99A(4):576-585.

[19]段永畅,田广永,郑伟,等.载NGF海藻酸凝胶-聚乳酸复合导管修复兔面神经缺损实验研究[J].中国临床解剖学杂志,2014,32(2): 189-191.

[20]张伟才,黄继锋,严琼娇,等.PNGF导管复合骨髓间充质干细胞修复大鼠12 mm坐骨神经缺损[J].中国临床解剖学杂志,2014, 32(3):325-329.

[21]Gao Z,Luo X,Li N,et al.Application of Amniotic ExtracellularMatrix Nerve Conduit with Biological Material in the Peripheral Nerve Defect in vivo.Adv Mater Res.2011; 322:173-176.

[22]Nakajima Y,Nishiura Y,Hara Y,et al.Simultaneous graduallengthening of proximal and distal nerve stumps for repair ofchronic peripheral nerve defect in rats.Hand Surg. 2012;17(1):1-11.

[23]钱婷玉,崔爱军,彭松,等.聚乳酸－羟基乙酸(PLGA)及其载药微球的制备研究[J].化工新型材料,2015,43(12):143-145.

[24]Qi R,Guo R,Zheng F,et al.Controlled release and antibacterial activity of antibiotic-loaded electrospun halloysite/ poly(lactic-co-glycolic acid)composite nanofibers.Colloids Surf B Biointerfaces.2013;110:148-155.

[25]郭立强.高分子量聚乳酸－羟基乙酸共聚物的合成[J].牡丹江师范学院学报(自然科学版),2014,87(2):13-15.

[26]Shin KC,Kim BS,Kim JH,et al.A facileprepration of highly interconnected macroporous PL GA scaf2folds by liquid2liquid phase separation. Polymer.2005;(46):3801-3808.

[27]Shen H,Hu X,Yang F,et al. Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide).Biomaterials. 2007; 28(29):4219-4230.

[28]Zhang ZJ,Li YJ,Liu XG,et al.Human umbilical cord blood stem cells and brain-derived neurotrophic factor for optic nerve injury: a biomechanical evaluation.Neural Regen Res.2015; 10(7):1134-1138.

[29]Wang Y,Li ZW,Luo M,et al.Biological conduits combining bone marrow mesenchymal stem cells and extracellular matrix to treat long-segment sciatic nerve defects.Neural Regen Res. 2015;10(6):965-971.

[30]张勇杰,金岩,聂鑫,等.组织工程周围神经修复坐骨神经缺损应用研究[J].中华神经外科疾病研究杂志,2004,3(2):141-144.

[31]汪玉海,金丽娟,高俊,等.脂肪干细胞复合PLGA 对骨质疏松骨折愈合后生物力学的影响[J].宁夏医科大学学报, 2013,3(3): 244-247.

[32]Kim WS,Vacanti JP,Cima L,et al. Cartilage engineered in predetermined shapes employing cell transplantation on synthetic biodegradable polymers.Plast Reeonstr Surg.1994; 94(2):233.

[33]李正伟,常笑语,李新颖,等.模拟坐骨神经损伤以聚乳酸－羟基乙酸管移植修复后蠕变特性的分析[J].生物医学工程研究,2015, 34(3):178-181.

[34]Peng SW,Li CW,Chiu IM,et al.Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane.Int J Nanomedicine. 2017; 12:421-432.

[35]Aldayel AM,Naguib YW,O'Mary HL,et al.Acid-Sensitive Sheddable PEGylated PLGA Nanoparticles Increase the Delivery of TNF-α siRNA in Chronic Inflammation Sites.Mol Ther Nucleic Acids.2016;5:e340.

[36]Bonaccorso A,Musumeci T,Carbone C,et al.Revisiting the role of sucrose in PLGA-PEG nanocarrier for potential intranasal delivery.Pharm Dev Technol. 2017:1-34. doi: 10.1080/10837450.2017.1287731. [Epub ahead of print]

[37]Liu X,Baldursdottir SG,Aho J,et al.Electrospinnability of Poly Lactic-co-glycolic Acid (PLGA): the Role of Solvent Type and Solvent Composition.Pharm Res. 2017. doi: 10.1007/s11095-017-2100-z. [Epub ahead of print]

[38]Landau S,Szklanny AA,Yeo GC,et al.Tropoelastin coated PLLA-PLGA scaffolds promote vascular network formation.Biomaterials.2017;122:72-82.

[39]Ni R,Muenster U,Zhao J,et al.Exploring polyvinylpyrrolidone in the engineering of large porous PLGA microparticles via single emulsion method with tunable sustained release in the lung: In vitro and in vivo characterization.J Control Release. 2017;249:11-22.

[40]Abamor ES.Antileishmanial activities of caffeic acid phenethyl ester loaded PLGA nanoparticles against Leishmania infantum promastigotes and amastigotes in vitro.Asian Pac J Trop Med. 2017;10(1):25-34.